My Account  |  Cart Contents  |  Checkout | My Wishlist   
Top » Catalog » Antibodies + Antigen » Human Proteins » S » Sox2 » GT15098
Quick Find
Use keywords to find the product you are looking for.
Advanced Search

View All Items
Shipping & Returns
Privacy Notice
Conditions of Use
Contact Us
Printable Catalog
Gift Voucher FAQ
Site Map
01.Goat anti Sox2
Goat anti Sox2

Type Antibody
Target Sox2
Immunogen Recombinant human SOX2 (a.a. 135-317)
Host/Source Goat
Type Polyclonal
Format Liquid
Pack Size 100 ug
Preparation Affinity purired
Buffer System Phosphate buffered saline with 5% trehlose
State Liquid
Storage Aliquot and store at -20ºC to -70ºC
IHC 5-10 ug/ml
Western Blot 5-10 ug/ml
ELISA 0.5 - 1.0 ug/ml
Storage Store at -20ºC to -70ºC for 6 months without detectable loss of activity. The antibody can be stored at 2 - 8ºC for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
This antibody has been used at 5-10ug/ml to detect human Sox2. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 0.1% Triton X-100, 1% BSA and 10% normal donkey serum at room temperature for 45 minutes. After blocking, sections were incubated with diluted primary antibody overnight at 4C followed by Rhodamine Red coupled anti-goat IgG antibody or another appropriate secondary antibody at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Wester Blot This antibody has been used at 0.1 - 0.2 ug/ml with the appropriate secondary reagents to detect human Sox2. The detection limit for recombinant human SOX2 is approximately 2ng/lane under non-reductin conditions

Product Information Sheet.
This product was added to our catalog on Monday 04 October, 2010.
Shopping Cart more
0 items
Sign in
E-mail address:


Password forgotten
Create an Account

All content and Images Copyright © 2024 Immune Systems Ltd
Copyright © 2000 - 2024 osCMax
Powered by osCMax v2.0.25