A custom service for preparing, dispensing and stabilising antibody or antigen into 96-well microwell ELISA plates using a high precision automated dispenser.
Dispensing and stabilising of antibody or antigen into 96 well microwell ELISA plates.
Microwell plate formats are either
(i) Multi-strip plates, or
(ii) Solid 96 well plates.
A wide range of microwell plates are stocked, with the option of specifying your preferred source.
There is no minimumn batch size. Hi performance automated coating is employed for 10 or more plates, due to a minimum dead volume required by the dispenser. Small batches are processed using calibrated multichannel pipettes.
We employ Stanard Operating Procedures which detail coating, blocking and stabilising conditions, and full batch records are maintained for auditing and certification.
Antibody or antigen is diluted in coating buffer
Antibody or antigen is dispensed into the microwell plates.
Plates are incubated to allow maximum coating
Uncoated plate surfaces are blocked with a Block /Stabilising solution to prevent non-specific binding and protect the antigen or antibody during long term storage.
Plates are vacuum dried and sealed in labelled zip-seal foil pouches containg silica gel to prevent degradation by moisture
ELISA plate formats Multi-strip or solid
Blocking agent Yes
Protein Stabiliser Yes
Inter batch precision Typically <4%
Intra batch precision Typically <6%
Presentation Security sealed re-sealable foil pouches with dessicant
Advantages of custom coating:
Low batch variation
No minimum batch size
Large batch size
Long term coating stability
Related products: Contract ELISA Kit Production
Call [UK +44] (0)1803 526556 to discuss your requirements.
Strict confidentiallity is observed.